elisa

MAK683 is an inhibitor of embryonic ectoderm development (EED) (IC50s: 59, 89, 26 nM in EED Alphascreen binding, LC-MS and ELISA assay).

Elisartan is a non-peptide pro-drug of angiotensin II AT1 receptor antagonist HN-12206. It also shows anti-hypertension activities.

SUVN-G3031 is a antagonist of histamine H3 receptor with potential for the treatment of cognitive impairment, dementia, attention deficit hyperactivity disorder, epilepsy, sleep disorders, obesity, schizophrenia, eating disorders, and pain.

Samelisant (SUVN-G3031) is a selective and inverse agonist of H3 receptor with Kis of 8.7 nM and 9.8 nM for human and rat. Samelisant has anticataplectic effects and can be used in studies about narcolepsy.

LF3 is a specific inhibitor of canonical Wnt signaling by disrupting the interaction between β-catenin and TCF4 (IC50 < 2 μM)

MAK683 hydrochloride embryonic ectodermal development (EED) inhibitor, also a potent PRC2 inhibitor, blocked cancer cell proliferation (IC50=1.014nM), with IC50 values of 59, 26, and 89 nM measured in EED Alphascreen, ELISA, and LC-MS assays, respectively.

Signal enhancer for use in western blotting, dot blotting and ELISA

CAY10760 is an inhibitor of the protein-protein interaction between RAD51 recombinase and BRCA2 (EC50= 19 μM in a cell-free competitive ELISA).1It decreases homologous recombination by 54% in wild-typeBRCA2-expressing BxPC-3 pancreatic cancer cells when used at a concentration of 20 μM. CAY10760 (20 μM) decreases proliferation of BxPC-3, as well as mutantBRCA2-expressing Capan-1, cancer cells when used alone or in combination with the poly(ADP-ribose) polymerase (PARP) inhibitor olaparib . 1.Bagnolini, G., Milano, D., Manerba, M., et al.Synthetic lethality in pancreatic cancer: Discovery of a new RAD51-BRCA2 small molecule disruptor that inhibits homologous recombination and synergizes with olaparibJ. Med. Chem.63(5)2588-2619(2020)

C2 Adamantanyl globotriaosylceramide (AdaGb3) is a bioactive sphingolipid and water-soluble form of globotriaosylceramide that contains an adamantanyl group in place of the fatty acyl chain. It inhibits Vero toxin binding to globotriaosylceramide in an ELISA assay when used at a concentration of 10 μM. AdaGb3 decreases cell surface expression of P-glycoprotein (P-gp) and reduces efflux of rhodamine 123 in MDCK cells. It also increases apical-to-basal transport of vinblastine in human intestinal C2BBe1 cells.

R-8507 is a small molecule antagonist of the TNF-α type 1 receptor (TNF-αRI). It inhibits the expression of intercellular adhesion molecule-1 (ICAM-1) induced by TNF-α and IL-1β with EC50 values of 2.45 and 3.79 μM, respectively, in an ELISA using A549 lung epithelial cells.{|Gururaja2007|} It also disrupts the interaction of the TNF-αRI with receptor interacting protein 1 (RIP1) and TNF-αR-associated death domain protein (TRADD), preventing internalization of the receptor complex.

TDRL-X80 is a potent inhibitor of the pigmented dry skin protein (XPA), inhibiting the DNA binding activity of XPA. It acts on single, double, and cisplatin-damaged DNA with IC50 values of 18, 20, and 29 μM in fluorescence polarization (FP) assays and 21, 39, and 28 μM in ELISA assays.

Blapsin B is a potent 14-3-3 inhibitor. Blapsin B is a naturally occurring compound isolated from the ethanol extract of the stink beetle, Blaps japanensis. Blapsin B potently inhibited 14-3-3 protein-protein interactions (PPIs) with IC(50) value of 10.0 μM as determined by an ELISA assay, and 2.5 μM in an FP assay, respectively. Blapsin B represent the first example of natural small-molecule 14-3-3 inhibitors.

VLVNTFCDSCIPKTYWNLGY TFA, an antigenic peptide of the M3 muscarinic receptor (M3R), is utilized in Enzyme-Linked Immunosorbent Assay (ELISA) [1].

PHD-IN-1 (compound 80) is a potent PHD2 inhibitor with an IC50 value of ≤5 nM and demonstrates EC50 values of 2.5 μM in EPO Elisa assays in both Caco2-HIF1a-HiBiT-clone-1 and Hep3B cell lines [1].

GluR23Y, a biologically active peptide, was utilized in an ELISA-based cell-surface assay to assess insulin-stimulated endocytosis of native AMPA receptors in cultured hippocampal neurons. GluR23Y effectively inhibited the insulin-induced decrease in receptor presence. The inhibition of insulin effects was achieved by introducing GluR23Y into neurons through fusion with the membrane transduction domain of HIV-1 TAT.

Thio-NAD, a thione-modified derivative of the signaling molecule and enzyme cofactor NAD+, can substitute for NAD+ as a cofactor in alkaline phosphatase (ALP) enzyme activity assays. It is utilized as a substrate in dual-enzyme cycling ELISA for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein, or surface glycoprotein, and live M. tuberculosis in patient sputum. Thio-NAD is characterized by its absorbance at 405 nm, distinguishing it from other cofactors.

HMBPP analog 1 (6e), a trifluoromethyl analog, acts as a potent inhibitor and a weak agonist in the HMBPP ELISA assay.

Horseradish Peroxidase, Biotin labeled (HRP-Biotin), is suitable for encapsulating DNA nanoflowers to develop a robust and streamlined ELISA platform that withstands harsh conditions.

Bovine Serum Albumin, Acetylated (Ac-BSA) is a well-characterized polypeptide with strong antigenic properties. It can elicit a significant immune response, thus validating the accuracy and reliability of experimental methods. Ac-BSA serves as a positive control in ELISA or WB experiments, particularly in assays involving acetylated lysine monoclonal or polyclonal antibodies. Additionally, it enhances encapsulation efficiency at low PLGA concentrations, with PLGA being a biocompatible, degradable, and controlled-release polymer used in drug delivery.

Sheep Immunoglobulin G (SheepIgG) is a serum immunoglobulin derived from sheep, useful as an immunolabeling and coating protein in various immunoassays, including ELISA.

Porcine Immunoglobulin G (PorcineIgG) is a porcine serum immunoglobulin utilized as an immunolabel and coating protein in various immunoassays, including ELISA.

Goat Immunoglobulin G (GoatIgG) is a goat serum immunoglobulin employed in various immunoassays, including ELISA, for use as an immunolabel and coating protein.

Rabbit Immunoglobulin G (RabbitIgG) is a serum immunoglobulin from rabbits, used for immunolabeling and as a coating protein in various immunoassays, such as ELISA.

Mouse Immunoglobulin G (MouseIgG) is a murine serum immunoglobulin utilized as an immunolabeling and coating protein in various immunoassays, including ELISA.