Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, IF

Reactivity: Human

Rabbit IgG

8J850

Human

1. IHC image of TMAH-01246 diluted at 1:40 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
2. IHC image of TMAH-01246 diluted at 1:40 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of NIH/3T3 cell with TMAH-01246 at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELISA, IHC, IF

IHC:1:20-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human VIM Protein

Human

7431

Neuroscience, Cancer, Developmental biology, Signal transduction, Stem cells