IL-12 Protein, Mouse, Recombinant (His)

Name: IL-12 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02350
Price: 68 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: p40, p35, Ll12a, interleukin 12A, Il-12p40, Il12p40, IL-12p35, Il-12b, Il-12a) Copy Product Info

Synonyms: p40, p35, Ll12a, interleukin 12A, Il-12p40, Il12p40, IL-12p35, Il-12b, Il-12a

Catalog No. TMPY-02350 Copy Product Info

Purity: 97.7%

IL-12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is P43431&P43432.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	-	In Stock
10 µg	$108	-	In Stock
20 µg	$177	7-10 days	7-10 days
50 µg	$346	-	In Stock
100 µg	$553	7-10 days	7-10 days
200 µg	$882	7-10 days	7-10 days
500 µg	$1,630	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:97.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-10 ng/mL.
Description	IL-12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is P43431&P43432.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P43431&P43432
Construction	A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Met 23
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.1 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	p40, p35, Ll12a, interleukin 12A, Il-12p40, Il12p40, IL-12p35, Il-12b, Il-12a

Chemical Properties

Molecular Weight

60.3 kDa (predicted); 28 & 47 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.