IL-12 Protein, Mouse, Recombinant (His)

IL-12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is P43431&P43432.

Pack Size	Availability	Price/USD
50 μg	In stock	$ 346.00
100 μg	5 days	$ 553.00
200 μg	5 days	$ 882.00
500 μg	5 days	$ 1,630.00

Pack Size

Availability

Price/USD

Quantity

50 μg

In stock

$ 346.00

100 μg

5 days

$ 553.00

200 μg

5 days

$ 882.00

500 μg

5 days

$ 1,630.00

Biological Information	1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-10 ng/mL.
Description	IL-12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is P43431&P43432.

Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P43431&P43432
Synonyms	Il-12b, p35, IL-12p35, Ll12a, Il-12a, interleukin 12A, Il12p40, Il-12p40, p40
Construction	The p35 subunit of Mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The p40 subunit of Mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	60.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.

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