MMP-2 Protein, Human, Recombinant

Name: MMP-2 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-01477
Price: 182 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: TBE-1, MONA, MMP-II, MMP-2, matrix metallopeptidase 2, CLG4A, CLG4) Copy Product Info

Synonyms: TBE-1, MONA, MMP-II, MMP-2, matrix metallopeptidase 2, CLG4A, CLG4

Catalog No. TMPY-01477 Copy Product Info

Purity: 95.5%

MMP-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 72 kDa and the accession number is A0A024R6R4.

Pack Size	Price	USA Stock	Global Stock
5 µg	$182	-	In Stock
10 µg	$303	-	In Stock
20 µg	$485	In Stock	In Stock
50 µg	$970	-	In Stock
100 µg	$1,485	-	In Stock
200 µg	$2,330	7-10 days	7-10 days
500 µg	$4,670	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.5%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is > 1,000 pmoles/min/µg. (Activation description: The proenzyme needs to be activated by APMA for an activated form) 2. Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 at 2 μg/ml (100 μl/well) can bind Human TIMP2 hFc, the EC50 of Human TIMP2 hFc is 6.0-30.0 ng/mL.
Description	MMP-2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 72 kDa and the accession number is A0A024R6R4.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P08253
Construction	A DNA sequence encoding the native human MMP2 (NP_004521.1) (Met 1-Cys 660) was expressed and purified. Predicted N terminal: Ala 30
Protein Purity	≥ 90 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 0.05% Brij-35, 150 mM NaCl, 5 mM CaCl2, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	TBE-1, MONA, MMP-II, MMP-2, matrix metallopeptidase 2, CLG4A, CLG4
Research Background	Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to a favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.

Chemical Properties

Molecular Weight

72 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.