Immobilized Human EGFR, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA. (QC Test) Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime). Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator® Prime).

EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.

Human

HEK293 Cells

PIG61,NISBD2,mENA,HER1,ERBB1,ERBB,epidermal growth factor receptor

Leu25-Ser645

> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.