Glypican 3/GPC3 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-06877

Glypican 3/GPC3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 64.05 kDa and the accession number is P51654-1.

Glypican 3/GPC3 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-06877

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$215	7-10 days	7-10 days
10 μg	$359	7-10 days	7-10 days
20 μg	$600	7-10 days	7-10 days
50 μg	$939	7-10 days	7-10 days
100 μg	$1,580	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Product Information

Biological Activity	Immobilized Recombinant Human bFGF / FGF2 Protein at 2 μg/mL (100 μl/well) can bind Recombinant Human Glypican 3/GPC3 Protein (His & AVI Tag), Biotinylated , the EC50 is 60-180 ng/mL.
Description	Glypican 3/GPC3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 64.05 kDa and the accession number is P51654-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P51654-1
Synonyms	SGBS1,SGBS,SGB,SDYS,OCI-5,MXR7,GTR2-2,Glypican 3,DGSX
Construction	Gln25-His559
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Molecular Weight	63.6 kDa (predicted); Due to glycosylation, the protein migrates to 42 kDa, 68-80 kDa based on Bis-Tris PAGE result.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in the lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue-dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceed both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy