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EphB2 Protein, Human, Recombinant (His)

TargetMol | SPR
Catalog No. TMPY-01067

EphB2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P29323-1.

EphB2 Protein, Human, Recombinant (His)

EphB2 Protein, Human, Recombinant (His)

TargetMol | SPR
Catalog No. TMPY-01067
EphB2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P29323-1.
Pack SizePriceAvailabilityQuantity
5 μg$687-10 days
10 μg$1087-10 days
20 μg$1787-10 days
50 μg$3597-10 days
100 μg$6967-10 days
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Product Information

Biological Activity
Immobilized Human EPHB2, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 2.2 ng/ml determined by ELISA (QC Test).
Immobilized Human EPHB2, His Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA.
Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Description
EphB2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P29323-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP29323-1
Synonyms
Tyro5,PCBC,Hek5,ERK,EPHT3,EPH receptor B2,EK5,DRT,CAPB
Construction
Val19-Leu543
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Molecular Weight59.2 kDa (predicted); 65-75 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

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