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Anti-PTPN11 Antibody (1U198)
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Catalog No. TMAH-01022
Anti-PTPN11 Antibody (1U198) is an antibody targeting PTPN11. Anti-PTPN11 Antibody (1U198) can be used in ELISA, WB, IHC.
Anti-PTPN11 Antibody (1U198)
Anti-PTPN11 Antibody (1U198)
😃Good
Catalog No. TMAH-01022
Anti-PTPN11 Antibody (1U198) is an antibody targeting PTPN11. Anti-PTPN11 Antibody (1U198) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PTPN11 Antibody (1U198) is an antibody targeting PTPN11. Anti-PTPN11 Antibody (1U198) can be used in ELISA, WB, IHC. |
| Ig Type | Rabbit IgG |
| Clone | 1U198 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate -All lanes: PTPN11 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 69, 53 kDa -Observed band size: 69 kDa 2. IHC image of TMAH-01022 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human SHP2 |
| Antigen Species | Human |
| Gene ID | 5781 |
| Uniprot ID | |
| Biology Area | Neuroscience, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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