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Anti-ATP7B Antibody (7M703)
😃Good
Catalog No. TMAH-00095
Alias WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B
Anti-ATP7B Antibody (7M703) is an antibody targeting ATP7B. Anti-ATP7B Antibody (7M703) can be used in ELISA, IF, FCM.
Anti-ATP7B Antibody (7M703)
Anti-ATP7B Antibody (7M703)
😃Good
Catalog No. TMAH-00095Alias WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B
Anti-ATP7B Antibody (7M703) is an antibody targeting ATP7B. Anti-ATP7B Antibody (7M703) can be used in ELISA, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IF, FCM Reactivity: Human |
| Alias | WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B |
| Ig Type | Rabbit IgG |
| Clone | 7M703 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Hela cell with TMAH-00095 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-00095 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, IF, FCM |
| Recommended Dose | IF:1:50-1:200; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome.; [Isoform 1]: Golgi apparatus membrane; Multi-pass membrane protein.; [Isoform 2]: Cytoplasm.; [WND/140 kDa]: Mitochondrion. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ATP7B Protein |
| Antigen Species | Human |
| Gene ID | 540 |
| Uniprot ID | |
| Biology Area | Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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