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SEMA4D Protein, Cynomolgus, Recombinant (His)

SEMA4D Protein, Cynomolgus, Recombinant (His)
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SEMA4D Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-01379
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. SEMA4D Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 80.1 kDa and the accession number is A0A2K5TZC9.
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Pack SizePriceAvailabilityQuantity
100 μg$3247-10 days
500 μg$1,3007-10 days
1 mg$2,1707-10 days
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Biological Description

Biological Information
Immobilized Cynomolgus Semaphorin 4D, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA.
Description
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. SEMA4D Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 80.1 kDa and the accession number is A0A2K5TZC9.
Species
Cynomolgus
Expression System
HEK293 Cells
TagC-His
Accession NumberA0A2K5TZC9
Synonyms
Semaphorin-4D,FLJ33485,FLJ46484,SEMA4D,FLJ39737,coll-4,FLJ34282,M-sema-G,SEMAJ,MGC169138,CD100,C9orf164,MGC169141
Construction
Phe24-Arg734
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight80.1 kDa (predicted). Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

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Keywords