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Anti-ABCB1 Antibody (7V919)
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Catalog No. TMAH-00002
Anti-ABCB1 Antibody (7V919) is an antibody targeting ABCB1. Anti-ABCB1 Antibody (7V919) can be used in ELISA, IHC, IF.
Anti-ABCB1 Antibody (7V919)
Anti-ABCB1 Antibody (7V919)
😃Good
Catalog No. TMAH-00002
Anti-ABCB1 Antibody (7V919) is an antibody targeting ABCB1. Anti-ABCB1 Antibody (7V919) can be used in ELISA, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-ABCB1 Antibody (7V919) is an antibody targeting ABCB1. Anti-ABCB1 Antibody (7V919) can be used in ELISA, IHC, IF. |
| Ig Type | Rabbit IgG |
| Clone | 7V919 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00002 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of HepG2 cell with TMAH-00002 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 583-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Application | |
| Recommended Dose | IHC:1:50-1:200; IF:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Apical cell membrane. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human ABCB1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer, Metabolism, Signal transduction, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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