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Anti-ABCB1 Antibody (7V919)

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Catalog No. TMAH-00002

Anti-ABCB1 Antibody (7V919) is an antibody targeting ABCB1. Anti-ABCB1 Antibody (7V919) can be used in ELISA, IHC, IF.

Anti-ABCB1 Antibody (7V919)

Anti-ABCB1 Antibody (7V919)

😃Good
Catalog No. TMAH-00002
Anti-ABCB1 Antibody (7V919) is an antibody targeting ABCB1. Anti-ABCB1 Antibody (7V919) can be used in ELISA, IHC, IF.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ABCB1 Antibody (7V919) is an antibody targeting ABCB1. Anti-ABCB1 Antibody (7V919) can be used in ELISA, IHC, IF.
Ig Type
Rabbit IgG
Clone
7V919
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00002 diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of HepG2 cell with TMAH-00002 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 583-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
ELISA, IHC, IF
Recommended Dose
IHC:1:50-1:200; IF:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Multi-pass membrane protein. Apical cell membrane.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundTranslocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human ABCB1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cancer, Metabolism, Signal transduction, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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