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Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454)
😃Good
Catalog No. TMAY-00001
Alias VH7183, Ighg1, Igh-4
Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454) is a Rabbit antibody targeting IgG1. Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454) can be used in ELISA.
Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454)
Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454)
😃Good
Catalog No. TMAY-00001Alias VH7183, Ighg1, Igh-4
Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454) is a Rabbit antibody targeting IgG1. Rabbit Anti-Mouse IgG1 Secondary Antibody (7L454) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $70 | 7-10 days | |
| 100 μL | $234 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA Reactivity: Mouse |
| Alias | VH7183, Ighg1, Igh-4 |
| Ig Type | Rabbit IgG |
| Clone | 7L454 |
| Reactivity | Mouse |
| Verified Activity | 1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG1 Secondary Antibody reacts only to mouse IgG1. No cross reactivity with mouse IgG2a, IgG2b, IgG3, IgM. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.1 μg/mL of Rabbit Anti-Mouse IgG1 Secondary Antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody. 2. Immunohistochemical analysis of PFA-fixed paraffin-embedded human stomach carcinoma tissue sections, with Claudin18.1 primary antibody (1:200) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG1 Secondary Antibody (1:1600) was used as the secondary antibody. 3. Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KLK15 primary antibody (1:60) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG1 Secondary Antibody (1:1600) was used as the secondary antibody. 4. Anti-Carboxypeptidase A monoclonal antibody at 1:1000 dilution. -Lane A: Recombinant Human Carboxypeptidase A Protein 100ng -Secondary -Rabbit Anti-Mouse IgG1/HRP at 1/1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
| Application | ELISA |
| Recommended Dose | ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IgG1 (rM IgG1; P01868-1Val98-Lys324,C102S). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Mouse IgG1-Fc Protein |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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