Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IF, FC

Reactivity: Human, Mouse

IgG1

4L497

Human, Mouse

1. Western Blot
-Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue
-All lanes: CD48 antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 28, 20 KDa
-Observed band size: 43 KDa
-Exposure time:5min
2. Western Blot
-Positive WB detected in: 20μg Raji whole cell lysate
CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 28, 20 KDa
-Observed band size: 43 KDa
-Exposure time:5min
3. Immunofluorescence staining of JK cells with TMAH-00208 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
4. Overlay Peak curve showing JK cells stained with TMAH-00208 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FC

Monoclonal

Unconjugated

Recombinant Protein: Human CD48 Protein (27-220AA)

Human

962

Immunology