CD40 Ligand Protein, Human, Recombinant (hFc & Avi), Biotinylated

Name: CD40 Ligand Protein, Human, Recombinant (hFc & Avi), Biotinylated
Brand: TargetMol
SKU: TMPY-05646
Rating: 4.5 (1 reviews)

(Synonyms: TRAP, TNFSF5, T-BAM, IMD3, IGM, HIGM1, hCD40L, gp39, CD40L, CD40 ligand, CD154) Copy Product Info

Synonyms: TRAP, TNFSF5, T-BAM, IMD3, IGM, HIGM1, hCD40L, gp39, CD40L, CD40 ligand, CD154

Catalog No. TMPY-05646 Copy Product Info

CD40 Ligand Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 44.7 kDa and the accession number is P29965.

Pack Size	Price	USA Stock	Global Stock
5 μg	$167	7-10 days	7-10 days
10 μg	$277	7-10 days	7-10 days
20 μg	$462	7-10 days	7-10 days
50 μg	$746	7-10 days	7-10 days
100 μg	$1,230	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human CD40 His at 2 μg/mL (100 μL/well) can bind Human CD40 Ligand (ECD, hFc & AVI Tag), Biotinylated, the EC50 is 5-40 ng/mL.
Description	CD40 Ligand Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 44.7 kDa and the accession number is P29965.
Species	Human
Expression System	HEK293 Cells
Tag	hFc, Avi
Accession Number	P29965
Construction	A DNA sequence encoding the human CD40LG (NP_000065.1) (Met113-Leu261) was expressed with a n-terminal Fc region of human IgG1 tagged AVI tag at the N-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly
Protein Purity	> 85 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	TRAP, TNFSF5, T-BAM, IMD3, IGM, HIGM1, hCD40L, gp39, CD40L, CD40 ligand, CD154
Research Background

Chemical Properties

Molecular Weight

44.7 kDa (predicted)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

TNFSF-5TNFSF 5IMD-3IMD 3HIGM-1HIGM 1CD-154CD 154

Related Tags: CD40 Ligand Protein, Human, Recombinant (hFc & Avi), Biotinylated chemical structure | CD40 Ligand Protein, Human, Recombinant (hFc & Avi), Biotinylated molecular weight