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Anti-Adenosine A3 Receptor Polyclonal Antibody is a Rabbit antibody targeting Adenosine A3 Receptor. Anti-Adenosine A3 Receptor Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-Adenosine A3 Receptor Polyclonal Antibody is a Rabbit antibody targeting Adenosine A3 Receptor. Anti-Adenosine A3 Receptor Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (TMAB-02322) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (TMAB-02322) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Measured by its binding ability in a indirect ELISA. Immobilized Human Adenosine A3 protein, His Tag at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human Adenosine A3 Antibody, the EC50 is 116.5 ng/mL. |
| Application | |
| Recommended Dose | IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | Recombinant Protein: human Adenosine receptor A3 protein |
| Antigen Species | Human |
| Gene Name | ADORA3 |
| Gene ID | |
| Protein Name | Adenosine receptor A3 |
| Uniprot ID | |
| Function | Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. |
| Molecular Weight | Theoretical: 35 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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