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Anti-Adenosine A3 Receptor Polyclonal Antibody

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Catalog No. TMAB-02322

Anti-Adenosine A3 Receptor Polyclonal Antibody is a Rabbit antibody targeting Adenosine A3 Receptor. Anti-Adenosine A3 Receptor Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA.

Anti-Adenosine A3 Receptor Polyclonal Antibody

Anti-Adenosine A3 Receptor Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-02322
Anti-Adenosine A3 Receptor Polyclonal Antibody is a Rabbit antibody targeting Adenosine A3 Receptor. Anti-Adenosine A3 Receptor Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Adenosine A3 Receptor Polyclonal Antibody is a Rabbit antibody targeting Adenosine A3 Receptor. Anti-Adenosine A3 Receptor Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (TMAB-02322) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (TMAB-02322) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Measured by its binding ability in a indirect ELISA. Immobilized Human Adenosine A3 protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human Adenosine A3 Antibody, the EC50 is 116.5 ng/mL.
Application
Recommended Dose
IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
Recombinant Protein: human Adenosine receptor A3 protein
Antigen Species
Human
Gene Name
ADORA3
Gene ID
Protein Name
Adenosine receptor A3
Uniprot ID
Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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