IL-10 Protein, Human, Recombinant (His & Avi)

Name: IL-10 Protein, Human, Recombinant (His & Avi)
Brand: TargetMol
SKU: TMPK-00056
Price: 79 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: TGIF, MGC126451, MGC126450, Interleukin-10, IL10A, IL-10, GVHDS, CSIF, B-TCGF) Copy Product Info

Synonyms: TGIF, MGC126451, MGC126450, Interleukin-10, IL10A, IL-10, GVHDS, CSIF, B-TCGF

Catalog No. TMPK-00056 Copy Product Info

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. IL-10 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 21.3 kDa and the accession number is P22301.

IL-10 Protein, Human, Recombinant (His & Avi)

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	-	In Stock
10 µg	$128	-	In Stock
20 µg	$209	7-10 days	7-10 days
50 µg	$415	7-10 days	7-10 days
100 µg	$698	-	In Stock
200 µg	$1,260	7-10 days	7-10 days
500 µg	$2,800	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized Human IL-10, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA. 2. Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay.
Description	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. IL-10 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 21.3 kDa and the accession number is P22301.
Species	Human
Expression System	HEK293 Cells
Tag	N-His-Avi
Accession Number	P22301
Construction	Ser19-Asn178
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	TGIF, MGC126451, MGC126450, Interleukin-10, IL10A, IL-10, GVHDS, CSIF, B-TCGF
Research Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.

Chemical Properties

Molecular Weight

21.3 kDa (predicted). Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.