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Anti-CD20 Antibody (4S869) is an antibody targeting CD20. Anti-CD20 Antibody (4S869) can be used in ELISA,IF,IHC.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
| Description | Anti-CD20 Antibody (4S869) is an antibody targeting CD20. Anti-CD20 Antibody (4S869) can be used in ELISA,IF,IHC. |
| Synonyms | MS4A-1, MS4A1, Membrane-spanning 4-domains subfamily A member 11, Leukocyte surface antigen Leu-16, CD20, Bp35, B-lymphocyte surface antigen B1 |
| Ig Type | Mouse IgG2a |
| Clone | 4S869 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00784 diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of Hela cell with TMAH-00784 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). 3. Immunofluorescence staining of MCF7 cell with TMAH-00784 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). |
| Application | |
| Recommended Dose | IHC:1:20-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor. |
| Tissue Specificity | Expressed on B-cells. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human MS4A1 Protein |
| Antigen Species | Human |
| Gene Name | MS4A1 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer, Immunology, Stem cells |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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