Antibody Type: Recombinant Monoclonal

Application: ELISA, IHC, IF

Reactivity: Human

Mouse IgG2a

4S869

Human

1. IHC image of TMAH-00784 diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of Hela cell with TMAH-00784 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
3. Immunofluorescence staining of MCF7 cell with TMAH-00784 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

ELISA, IHC, IF

IHC:1:20-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human MS4A1 Protein

Human

931

Cancer, Immunology, Stem cells