HGFR/c-Met Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-05607

HGFR/c-Met Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 104.9 kDa and the accession number is A0A024R759.

HGFR/c-Met Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-05607

Pack Size	Price	Availability	Quantity
20 μg	$462	7-10 days
100 μg	$1,070	7-10 days

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Product Information

Biological Activity	Immobilized Recombinant Human HGF / Hepatocyte Growth Factor Protein at 2μg/mL (100μL/well) can bind Recombinant Human c-Met / HGFR Protein (His & AVI Tag), Biotinylated , the EC50 is 10-30 ng/mL.
Description	HGFR/c-Met Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 104.9 kDa and the accession number is A0A024R759.
Species	Human
Expression System	HEK293 Cells
Tag	His, Avi
Accession Number	P08581
Synonyms	RCCP2,MET proto-oncogene, receptor tyrosine kinase,HGFR,DFNB97,c-Met,AUTS9
Construction	A DNA sequence encoding the human MET (NP_000236.2) (Met1-Thr932) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Glu 25
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.
Molecular Weight	104.9 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy