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Anti-BCL-XL Antibody (3V974)
😃Good
Catalog No. TMAY-00850
Alias Bcl-XL, bcl-x, BclX, BCL2-like 1, bcl2-L-1, Bcl2l, Bcl(X)L
Anti-BCL-XL Antibody (3V974) is a Mouse antibody targeting BCL-XL. Anti-BCL-XL Antibody (3V974) can be used in ELISA.
Anti-BCL-XL Antibody (3V974)
Anti-BCL-XL Antibody (3V974)
😃Good
Catalog No. TMAY-00850Alias Bcl-XL, bcl-x, BclX, BCL2-like 1, bcl2-L-1, Bcl2l, Bcl(X)L
Anti-BCL-XL Antibody (3V974) is a Mouse antibody targeting BCL-XL. Anti-BCL-XL Antibody (3V974) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA Reactivity: Human |
| Alias | Bcl-XL, bcl-x, BclX, BCL2-like 1, bcl2-L-1, Bcl2l, Bcl(X)L |
| Ig Type | Mouse IgG1 |
| Clone | 3V974 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Purified anti-Human BCL2L1 (Bcl-xL) on HeLa cells. HeLa cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-BCL2L1 (Bcl-xL) (Bold line hisgram), then stained with a FITC-conjugated second step antibody. To demonstrate specificity of staining the binding of Anti-BCL-XL Antibody was blocked by the preincubation of the purified antibody with molar excess of recombinant human BCL2L1 (Bcl-xL) (5 µg) for 1 hour (dashed line hisgram). |
| Application | ELISA |
| Recommended Dose | ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Bcl-XL / BCL2L1 (rh Bcl-XL / BCL2L1; TMPY-02431; NP_612815.1; Met 1-Arg 212). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Bcl-XL / BCL2L1 protein (TMPY-02431) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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