Anti-C-jun Polyclonal Antibody is a Rabbit antibody targeting C-jun. Anti-C-jun Polyclonal Antibody can be used in FCM,ELISA.

IgG

Human

1. Measured by its binding ability in a indirect ELISA. Immobilized Human C-jun protein, His & MBP Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human C-jun Antibody, the EC50 is 180.9 ng/mL.
2. Measured by its binding ability in a indirect ELISA. Immobilized Human C-jun protein, His & MBP Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human C-jun Antibody, the EC50 is 27.15 ng/mL.
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-C-jun antibody (TMAB-04374)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human C-jun protein

Human

JUN

Transcription factor AP-1

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.