Siglec-2/CD22 Protein, Human, Recombinant (His)

Name: Siglec-2/CD22 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02034
Rating: 4.5 (1 reviews)

(Synonyms: SIGLEC-2, SIGLEC2, CD22 molecule) Copy Product Info

Synonyms: SIGLEC-2, SIGLEC2, CD22 molecule

Catalog No. TMPY-02034 Copy Product Info

Purity: 97.6%

Siglec-2/CD22 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76.6 kDa and the accession number is P20273-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$35	7-10 days	7-10 days
10 µg	$53	7-10 days	7-10 days
20 µg	$83	-	In Stock
50 µg	$156	-	In Stock
100 µg	$259	-	In Stock
200 µg	$459	7-10 days	7-10 days
500 µg	$971	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:97.6%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized human CD22 at 0.02 μg/ml (100uL/well) can bind anti-human CD22 Mab with a linear range of 0.2-50 ng/ml.
Description	Siglec-2/CD22 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76.6 kDa and the accession number is P20273-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P20273-1
Construction	A DNA sequence encoding the human CD22 isoform-beta (P20273-1) extracellular domain (Met 1-Arg 687) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 20
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	SIGLEC-2, SIGLEC2, CD22 molecule
Research Background	CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

76.6 kDa (predicted); 108 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.