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Anti-Caspase-3 Antibody (4L394)

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Catalog No. TMAH-00141
Alias Yama, SCA-1, CPP32B, CPP32, apopain

Anti-Caspase-3 Antibody (4L394) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (4L394) can be used in ELISA, WB, IHC.

Anti-Caspase-3 Antibody (4L394)

Anti-Caspase-3 Antibody (4L394)

😃Good
Catalog No. TMAH-00141Alias Yama, SCA-1, CPP32B, CPP32, apopain
Anti-Caspase-3 Antibody (4L394) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (4L394) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human
AliasYama, SCA-1, CPP32B, CPP32, apopain
Ig Type
Rabbit IgG
Clone
4L394
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 cell lysate
-All lanes: CASP3 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 32 kDa
-Observed band size: 32, 55, 130 kDa
2. IHC image of TMAH-00141 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human active + pro Caspase 3
Antigen Species
Human
Gene ID
836
Uniprot ID
Biology Area
Cancer, Cell biology, Metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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