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MSLN/Mesothelin Protein, Human, Recombinant

(Synonyms: SMRP, MPF, Mesothelin) Copy Product Info

Synonyms: SMRP, MPF, Mesothelin

Catalog No. TMPY-00132 Copy Product Info
Mesothelin Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 32.4 kDa and the accession number is Q13421-3.

Batch Information

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Purity:SDS-PAGE: 97.3%; SEC-HPLC: 98.9%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Recombinant Human MSLN/Mesothelin Protein (Cat#TMPY-00132) at 2 μg/mL (100 μL/well) can bind Anti-MSLN Antibody, human IgG1, the EC50 is 7.5-24 ng/mL.
Description
Mesothelin Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 32.4 kDa and the accession number is Q13421-3.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberQ13421-3
ConstructionA DNA sequence encoding the human MSLN (NP_005814.2) (Glu296-Gly580) was expressed with two additional amino acids (Gly & Pro) at the N-terminus. Predicted N terminal: Gly
Protein Purity
≥ 85 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
MSLN/Mesothelin Protein, Human, RecombinantMSLN/Mesothelin Protein, Human, Recombinant
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsSMRP, MPF, Mesothelin
Research Background
Chemical Properties
Molecular Weight32.4 kDa (predicted); (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: MSLN/Mesothelin Protein, Human, Recombinant chemical structure | MSLN/Mesothelin Protein, Human, Recombinant molecular weight