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Anti-DOT1L Antibody (6U296)
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Catalog No. TMAH-00361
Alias KMT4, DOT1-like histone H3K79 methyltransferase, DOT1
Anti-DOT1L Antibody (6U296) is an antibody targeting DOT1L. Anti-DOT1L Antibody (6U296) can be used in ELISA, WB.
Anti-DOT1L Antibody (6U296)
Anti-DOT1L Antibody (6U296)
😃Good
Catalog No. TMAH-00361Alias KMT4, DOT1-like histone H3K79 methyltransferase, DOT1
Anti-DOT1L Antibody (6U296) is an antibody targeting DOT1L. Anti-DOT1L Antibody (6U296) can be used in ELISA, WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB Reactivity: Human |
| Alias | KMT4, DOT1-like histone H3K79 methyltransferase, DOT1 |
| Ig Type | Rabbit IgG |
| Clone | 6U296 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate -All lanes: DOT1L antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 165 kDa -Observed band size: 165 kDa 2. Immunofluorescence staining of Hela cell with TMAH-00361 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Application | ELISA, WB |
| Recommended Dose | WB:1:500-1:5000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human KMT4 / Dot1L |
| Antigen Species | Human |
| Gene ID | 84444 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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