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Anti-DOT1L Antibody (6U296) (Synonyms: KMT4, DOT1-like histone H3K79 methyltransferase, DOT1)

Catalog No. TMAH-00361 Copy Product Info
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Anti-DOT1L Antibody (6U296) is an antibody targeting DOT1L. Anti-DOT1L Antibody (6U296) can be used in ELISA,WB.

Anti-DOT1L Antibody (6U296)

Copy Product Info
🥰Excellent
Catalog No. TMAH-00361
Synonyms KMT4, DOT1-like histone H3K79 methyltransferase, DOT1

Anti-DOT1L Antibody (6U296) is an antibody targeting DOT1L. Anti-DOT1L Antibody (6U296) can be used in ELISA,WB.

Anti-DOT1L Antibody
(6U296)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2077-10 days7-10 days
100 μL$3487-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-DOT1L Antibody (6U296) is an antibody targeting DOT1L. Anti-DOT1L Antibody (6U296) can be used in ELISA,WB.
SynonymsKMT4, DOT1-like histone H3K79 methyltransferase, DOT1
Ig Type
Rabbit IgG
Clone
6U296
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate
-All lanes: DOT1L antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 165 kDa
-Observed band size: 165 kDa
2. Immunofluorescence staining of Hela cell with TMAH-00361 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
Recommended Dose
WB:1:500-1:5000.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundHistone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human KMT4 / Dot1L
Antigen Species
Human
Gene Name
DOT1L
Gene ID
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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