Shopping Cart
Remove All
  • TargetMol
    Your shopping cart is currently empty

EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated

(Synonyms: TROP1, TACSTD1, TACST-1, MOC31, MK1, MIC18, KSA, HNPCC8, gp40, ESA, EpCAM, EGP40, EGP314, EGP-2, EGP, DIAR5, CD326, ACSTD1) Copy Product Info

Synonyms: TROP1, TACSTD1, TACST-1, MOC31, MK1, MIC18, KSA, HNPCC8, gp40, ESA, EpCAM, EGP40, EGP314, EGP-2, EGP, DIAR5, CD326, ACSTD1

Catalog No. TMPK-00205 Copy Product Info
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 30.1 kDa and the accession number is P16422.

Product Introduction

Bioactivity
Bioactivity
Immobilized Anti-EpCAM Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 14.4ng/ml determined by ELISA.
Description
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 30.1 kDa and the accession number is P16422.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP16422
ConstructionGln24-Lys265
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTROP1, TACSTD1, TACST-1, MOC31, MK1, MIC18, KSA, HNPCC8, gp40, ESA, EpCAM, EGP40, EGP314, EGP-2, EGP, DIAR5, CD326, ACSTD1
Research Background
Chemical Properties
Molecular Weight30.1 kDa (predicted). Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated chemical structure | EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated molecular weight