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Anti-CD8 alpha Antibody (1R433)
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Catalog No. TMAH-00229
Alias p32, OKT8 T-cell antigen, MAL, Lyt-2, Leu2, CD8a molecule, CD8a, CD8 α/CD8A, CD8
Anti-CD8 alpha Antibody (1R433) is an antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (1R433) can be used in ELISA, WB, IHC, IP.
Anti-CD8 alpha Antibody (1R433)
Anti-CD8 alpha Antibody (1R433)
😃Good
Catalog No. TMAH-00229Alias p32, OKT8 T-cell antigen, MAL, Lyt-2, Leu2, CD8a molecule, CD8a, CD8 α/CD8A, CD8
Anti-CD8 alpha Antibody (1R433) is an antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (1R433) can be used in ELISA, WB, IHC, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IP Reactivity: Human |
| Alias | p32, OKT8 T-cell antigen, MAL, Lyt-2, Leu2, CD8a molecule, CD8a, CD8 α/CD8A, CD8 |
| Ig Type | Rabbit IgG |
| Clone | 1R433 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate -All lanes: CD8A antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 26, 22, 31 kDa -Observed band size: 31 kDa 2. IHC image of TMAH-00229 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00229 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunoprecipitating CD8A in HepG2 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00229 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2:TMAH-00229(3µg)+ HepG2 whole cell lysate(500µg) -Lane 3: HepG2 whole cell lysate (10µg) |
| Application | ELISA, WB, IHC, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human CD8 |
| Antigen Species | Human |
| Gene ID | 925 |
| Uniprot ID | |
| Biology Area | Immunology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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