B7-2/CD86 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00882 Copy Product Info

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 52 kDa and the accession number is P42081-1.

B7-2/CD86 Protein, Human, Recombinant (hFc)

Catalog No. TMPK-00882

Copy Product Info

B7-2/CD86 Protein, Human, Recombinant (hFc)

Pack Size	Price	USA Stock	Global Stock
5 μg	$39	7-10 days	7-10 days
10 μg	$60	7-10 days	7-10 days
20 μg	$95	7-10 days	7-10 days
50 μg	$182	7-10 days	7-10 days
100 μg	$302	7-10 days	7-10 days
200 μg	$547	7-10 days	7-10 days
500 μg	$1,210	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Information

Biological Activity	Immobilized Human CTLA-4, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA.
Description	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 52 kDa and the accession number is P42081-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P42081-1
Synonyms	MGC34413,LAB72,FUN-1,CD86 molecule,CD86,CD28LG2,BU63,B7-2 antigen,B7-2,B72,B70
Construction	Leu26-Pro247
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	52 kDa (predicted). Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.