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Anti-HGFR/c-Met Polyclonal Antibody
😃Good
Catalog No. TMAB-00853
Anti-HGFR/c-Met Polyclonal Antibody is a Rabbit antibody targeting HGFR/c-Met. Anti-HGFR/c-Met Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-HGFR/c-Met Polyclonal Antibody
Anti-HGFR/c-Met Polyclonal Antibody
😃Good
Catalog No. TMAB-00853
Anti-HGFR/c-Met Polyclonal Antibody is a Rabbit antibody targeting HGFR/c-Met. Anti-HGFR/c-Met Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,ELISA Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Mouse Lung tissue lysates Lane 4: Rat Kidney tissue lysates Lane 5: Rat Stomach tissue lysates Lane 6: Rat Lung tissue lysates Primary: Anti-MET (TMAB-00853) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33/123/153 kDa Observed band size: 145 kDa 2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-C-Met Polyclonal Antibody, Unconjugated (TMAB-00853) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: Human gastric cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Met (c Met) Polyclonal Antibody, Unconjugated (TMAB-00853) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Tissue/cell: mouse intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-C-Met Polyclonal Antibody, Unconjugated (TMAB-00853) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. Isoform 3: Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016] |
| Immunogen | KLH conjugated synthetic peptide: mouse MET |
| Antigen Species | Mouse |
| Gene ID | 17295 |
| Uniprot ID | |
| Biology Area | Growth factor receptors,Proto-oncogenes,Receptor Tyrosine Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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