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Anti-KIFAP3 Antibody (2F836) (Alias: Smg GDS-associated protein, SMAP, Kinesin-associated protein 3, KIFAP...)

Catalog No. TMAH-00666 Copy Product Info
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Anti-KIFAP3 Antibody (2F836) is an antibody targeting KIFAP3. Anti-KIFAP3 Antibody (2F836) can be used in ELISA,IF,IHC,WB.

Anti-KIFAP3 Antibody (2F836)

Copy Product Info
🥰Excellent
Catalog No. TMAH-00666
Alias Smg GDS-associated protein, SMAP, Kinesin-associated protein 3, KIFAP 3, KIF3AP, KAP-3, KAP3

Anti-KIFAP3 Antibody (2F836) is an antibody targeting KIFAP3. Anti-KIFAP3 Antibody (2F836) can be used in ELISA,IF,IHC,WB.

Anti-KIFAP3 Antibody
(2F836)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2087-10 days7-10 days
100 μL$3497-10 days7-10 days
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-KIFAP3 Antibody (2F836) is an antibody targeting KIFAP3. Anti-KIFAP3 Antibody (2F836) can be used in ELISA,IF,IHC,WB.
SynonymsSmg GDS-associated protein, SMAP, Kinesin-associated protein 3, KIFAP 3, KIF3AP, KAP-3, KAP3
Ig Type
Rabbit IgG
Clone
2F836
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Hela whole cell lysate
-All lanes: KIFAP3 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 92, 87, 83 kDa
-Observed band size: 72-95 kDa
2. IHC image of TMAH-00666 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of PC-3 cell with TMAH-00666 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 552-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
Recommended Dose
WB:1:500-1:2000; IHC:1:50-1:200; IF:1:50-1:200.
Antibody Type
Monoclonal
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundInvolved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human KIFAP3
Antigen Species
Human
Gene Name
KIFAP3
Gene ID
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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