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Anti-KIFAP3 Antibody (2F836)
😃Good
Catalog No. TMAH-00666
Alias Smg GDS-associated protein, SMAP, Kinesin-associated protein 3, KIFAP 3, KIF3AP, KAP-3, KAP3
Anti-KIFAP3 Antibody (2F836) is an antibody targeting KIFAP3. Anti-KIFAP3 Antibody (2F836) can be used in ELISA, WB, IHC, IF.
Anti-KIFAP3 Antibody (2F836)
Anti-KIFAP3 Antibody (2F836)
😃Good
Catalog No. TMAH-00666Alias Smg GDS-associated protein, SMAP, Kinesin-associated protein 3, KIFAP 3, KIF3AP, KAP-3, KAP3
Anti-KIFAP3 Antibody (2F836) is an antibody targeting KIFAP3. Anti-KIFAP3 Antibody (2F836) can be used in ELISA, WB, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | 197 € | 7-10 days | |
| 100 μL | 331 € | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-KIFAP3 Antibody (2F836) is an antibody targeting KIFAP3. Anti-KIFAP3 Antibody (2F836) can be used in ELISA, WB, IHC, IF. |
| Alias | Smg GDS-associated protein, SMAP, Kinesin-associated protein 3, KIFAP 3, KIF3AP, KAP-3, KAP3 |
| Ig Type | Rabbit IgG |
| Clone | 2F836 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate -All lanes: KIFAP3 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 92, 87, 83 kDa -Observed band size: 72-95 kDa 2. IHC image of TMAH-00666 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of PC-3 cell with TMAH-00666 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 552-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Application | ELISA, WB, IHC, IF |
| Recommended Dose | WB:1:500-1:2000; IHC:1:50-1:200; IF:1:50-1:200. |
| Antibody Type | Monoclonal |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human KIFAP3 |
| Antigen Species | Human |
| Gene ID | 22920 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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