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LILRB1/CD85j/ILT2 Domain1 & 2 Protein, Human, Recombinant (His)

(Synonyms: XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J) Copy Product Info

Synonyms: XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J

Catalog No. TMPK-01111 Copy Product Info
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. LILRB1/CD85j/ILT2 Domain1 & 2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 23.1 kDa and the accession number is Q8NHL6-1.

Product Introduction

Bioactivity
Bioactivity
1. Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). 2. Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9μM as determined in SPR assay.
Description
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. LILRB1/CD85j/ILT2 Domain1 & 2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 23.1 kDa and the accession number is Q8NHL6-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberQ8NHL6-1
ConstructionGly24-Gly221
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsXXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J
Research Background
Chemical Properties
Molecular Weight23.1 kDa (predicted). Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: LILRB1/CD85j/ILT2 Domain1 & 2 Protein, Human, Recombinant (His) chemical structure | LILRB1/CD85j/ILT2 Domain1 & 2 Protein, Human, Recombinant (His) molecular weight