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Anti-Nucleoprotein/NTD Antibody (5Y349)

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Catalog No. TMAH-00834
Alias Nucleocapsid, NP, NC

Anti-Nucleoprotein/NTD Antibody (5Y349) is a Mouse antibody targeting Nucleoprotein/NTD. Anti-Nucleoprotein/NTD Antibody (5Y349) can be used in ELISA, IHC.

Anti-Nucleoprotein/NTD Antibody (5Y349)

Anti-Nucleoprotein/NTD Antibody (5Y349)

😃Good
Catalog No. TMAH-00834Alias Nucleocapsid, NP, NC
Anti-Nucleoprotein/NTD Antibody (5Y349) is a Mouse antibody targeting Nucleoprotein/NTD. Anti-Nucleoprotein/NTD Antibody (5Y349) can be used in ELISA, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: ELISA, IHC

Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
AliasNucleocapsid, NP, NC
Ig Type
IgG1
Clone
5Y349
Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Verified Activity
1. The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 15.36 to 23.97 ng/ml.
2. Western Blot
-Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli at 40ng, 20ng, 10ng, 5ng
-All lanes N antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 48 KDa
-Observed band size: 55 KDa
3. Western Blot
-Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli at 100ng
-All lanes N antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 18 KDa
-Observed band size: 18 KDa
4. The Binding Activity of SARS-CoV-2-N (NTD) Antibody with SARS-CoV-2-N (NTD) Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N Antibody, the EC50 is 30.98 to 40.70 ng/ml.
5. The Binding Activity of N protein with N Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Antibody, the EC50 of N Antibody.
6. The Binding Activity of N protein with N Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Antibody, the EC50 of N Antibody.
7. The Binding Activity of N protein with N Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Antibody, the EC50 of N Antibody.
Application
ELISA, IHC
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationVirion. Host cytoplasm.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Purity>95%
Research BackgroundPackages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human Novel Coronavirus NucleoProtein (N) (1-419aa)
Antigen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Uniprot ID
Biology Area
Others
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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