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Anti-Nucleoprotein/NTD Antibody (5Y349) is a Mouse antibody targeting Nucleoprotein/NTD. Anti-Nucleoprotein/NTD Antibody (5Y349) can be used in ELISA, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: ELISA, IHC Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Alias | Nucleocapsid, NP, NC |
Ig Type | IgG1 |
Clone | 5Y349 |
Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Verified Activity | 1. The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 15.36 to 23.97 ng/ml. 2. Western Blot -Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli at 40ng, 20ng, 10ng, 5ng -All lanes N antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 48 KDa -Observed band size: 55 KDa 3. Western Blot -Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli at 100ng -All lanes N antibody at 1:2000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 18 KDa -Observed band size: 18 KDa 4. The Binding Activity of SARS-CoV-2-N (NTD) Antibody with SARS-CoV-2-N (NTD) Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N Antibody, the EC50 is 30.98 to 40.70 ng/ml. 5. The Binding Activity of N protein with N Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Antibody, the EC50 of N Antibody. 6. The Binding Activity of N protein with N Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Antibody, the EC50 of N Antibody. 7. The Binding Activity of N protein with N Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Antibody, the EC50 of N Antibody. |
Application | ELISA, IHC |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Virion. Host cytoplasm. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Purity | >95% |
Research Background | Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human Novel Coronavirus NucleoProtein (N) (1-419aa) |
Antigen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) |
Uniprot ID | |
Biology Area | Others |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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