CD21 Protein, Human, Recombinant (His)

Catalog No. TMPK-00842

CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.

CD21 Protein, Human, Recombinant (His)

Catalog No. TMPK-00842

Pack Size	Price	Availability
5 μg	$51	7-10 days
10 μg	$80	7-10 days
20 μg	$129	7-10 days
50 μg	$248	7-10 days
100 μg	$418	7-10 days
200 μg	$757	7-10 days
500 μg	$1,670	7-10 days

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Product Information

Biological Activity	Immobilized Human CD21, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
Description	CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P20023-1
Synonyms	SLEB9,EBV receptor,CVID7,CR2,CD21,C3DRSLEB9,C3DR
Construction	Ile21-Arg971
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	105.3 kDa (predicted). Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.