FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

Name: FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated
Brand: TargetMol
SKU: TMPY-05650
Rating: 4.5 (1 reviews)

(Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha) Copy Product Info

Synonyms: Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

Catalog No. TMPY-05650 Copy Product Info

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.

Pack Size	Price	USA Stock	Global Stock
5 µg	$109	7-10 days	7-10 days
10 µg	$178	7-10 days	7-10 days
20 µg	$296	-	In Stock
50 µg	$478	7-10 days	7-10 days
100 µg	$690	7-10 days	7-10 days
200 µg	$1,180	7-10 days	7-10 days
500 µg	$2,380	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 98.3%; SEC-HPLC: 91.3%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized Anti-CD20 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus FCGRT&B2M Heterodimer Protein (His & AVI Tag), Biotinylated, the EC50 is 80-240 ng/mL. 2. Loaded Biotinylated Cynomolgus FcRn recombinant protein ( His & Avi Tag ) on SA Biosensor, can bind IgG1 Fc with an affinity constant of 0.08 μM as determined in a BLI assay. 3. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 90% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.
Description	FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	His, Avi
Accession Number	Q8SPV9&Q8SPW0
Construction	A DNA sequence encoding the cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

Chemical Properties

Molecular Weight

45.3 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated chemical structure | FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated molecular weight