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TIGIT Protein, Human, Recombinant (hFc)

Catalog No. TMPY-04970
Synonyms: WUCAM, VSTM3, VSIG9, T cell immunoreceptor with Ig and ITIM domains

TIGIT Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
TIGIT Protein, Human, Recombinant (hFc)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 198.00
100 μg 5 days $ 351.00
200 μg 5 days $ 621.00
500 μg 5 days $ 1,320.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information 1. Immobilized human CD155a-His at 10μg/mL (100μL/well) can bind human TIGIT-Fc, the EC50 of human TIGIT-Fc is 0.03-0.2μg/mL. 2.Loaded Recombinant Human TIGIT Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human CD155/PVR Protein, His Tag with an affinity constant of 0.18 uM as determined in BLI assay (Routinely tested).

Description TIGIT Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.
Species Human
Expression System HEK293 Cells
Tag C-hFc
Accession Number Q495A1-1
Synonyms WUCAM, VSTM3, VSIG9, T cell immunoreceptor with Ig and ITIM domains
Construction The Human TIGIT (NP_776160.2) (Met1-Pro141) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity ≥ 95% as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight 39.2 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy

References and Literature

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Keywords

TIGIT Protein, Human, Recombinant (hFc) WUCAM VSIG 9 VSTM 3 VSTM3 VSTM-3 VSIG9 T cell immunoreceptor with Ig and ITIM domains VSIG-9 recombinant recombinant-proteins proteins protein

 

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