TIGIT Protein, Human, Recombinant (hFc)

Name: TIGIT Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-04970
Price: 42 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: WUCAM, VSTM3, VSIG9, T cell immunoreceptor with Ig and ITIM domains) Copy Product Info

Synonyms: WUCAM, VSTM3, VSIG9, T cell immunoreceptor with Ig and ITIM domains

Catalog No. TMPY-04970 Copy Product Info

Purity: 95.7%

TIGIT Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$42	-	In Stock
10 µg	$65	-	In Stock
20 µg	$98	-	In Stock
50 µg	$198	-	In Stock
100 µg	$351	7-10 days	7-10 days
200 µg	$621	7-10 days	7-10 days
500 µg	$1,320	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1. Immobilized human CD155a-His at 10μg/mL (100μL/well) can bind human TIGIT-Fc, the EC50 of human TIGIT-Fc is 0.03-0.2μg/mL. 2.Loaded Recombinant Human TIGIT Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human CD155/PVR Protein, His Tag with an affinity constant of 0.18 uM as determined in BLI assay (Routinely tested).
Description	TIGIT Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 39.2 kDa and the accession number is Q495A1-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q495A1-1
Construction	A DNA sequence encoding the human TIGIT (NP_776160.2) (Met1-Pro141) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Met 22
Protein Purity	≥ 95% as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	WUCAM, VSTM3, VSIG9, T cell immunoreceptor with Ig and ITIM domains
Research Background	TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

39.2 kDa (predicted); 45 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.