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Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335)
😃Good
Catalog No. TMAY-02251
Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335) is a Mouse antibody targeting KLH/Keyhole Limpet Hemocyanin. Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335) can be used in WB,ELISA.
Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335)
Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335)
😃Good
Catalog No. TMAY-02251
Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335) is a Mouse antibody targeting KLH/Keyhole Limpet Hemocyanin. Anti-KLH/Keyhole Limpet Hemocyanin Antibody (6H335) can be used in WB,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA Reactivity: other |
| Ig Type | Mouse IgG1 |
| Clone | 6H335 |
| Reactivity | other |
| Application | WB,ELISA |
| Recommended Dose | WB: his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect KLH in WB.; ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant KLH (Keyhole Limpet Hemocyanin). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | KLH (Keyhole Limpet Hemocyanin) is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is used extensively as a carrier protein in the production of antibodies for research, biotechnology, and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins, and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier proteins for this purpose. It is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and the abundance of lysine residues for coupling haptens, allows a high hapten: carrier protein ratio increasing the likelihood of generating hapten-specific antibodies. KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory. A high-quality KLH preparation with a clear opalescent blue color is the best indicator of KLH solubility. KLH is being tested as a therapeutic vaccine for a variety of cancers, including non-Hodgkins lymphoma, cutaneous melanoma, breast, and bladder cancer. These vaccines use specific tumor-associated antigens (Haptens) conjugated to KLH to stimulate the body’s immune system to generate anti-tumor immune responses which can destroy tumor cells. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: KLH protein |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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