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Anti-CD14 Antibody (8B111) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (8B111) can be used in WB,ELISA,ELISA(Det).
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $93 | 7-10 days | |
100 μL | $269 | 7-10 days |
Description | Anti-CD14 Antibody (8B111) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (8B111) can be used in WB,ELISA,ELISA(Det). |
Alias | CD14 molecule, CD14 |
Ig Type | Monoclonal Rabbit IgG |
Clone | 8B111 |
Reactivity | Mouse |
Verified Activity | Anti-CD14 rabbit monoclonal antibody at 1:500 dilution. -Lane A: SW480 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:40 kDa. -Observed band size:63 kDa |
Application | WB,ELISA,ELISA(Det) |
Recommended Dose | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; ELISA(Det): 1:5000-1:50000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 extracellular domain (rM CD14; TMPY-00698; NP_033971.1; Met 1-Pro 345). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns. |
Conjucates | Unconjugated |
Immunogen | Recombinant Mouse CD14 protein (TMPY-00698) |
Antigen Species | Mouse |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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