Immobilized Biotinylated Human CD73, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5 ng/ml determined by ELISA.

CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is P21589-1.

Human

HEK293 Cells

NTE,NT5,NT,eNT,eN,E5NT,CD73,CALJA,5'-nucleotidase, ecto (CD73)

Trp27-Lys547

> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.