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Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $213 | 7-10 days | 7-10 days | |
| 10 μg | $357 | 7-10 days | 7-10 days | |
| 20 μg | $600 | 7-10 days | 7-10 days | |
| 50 μg | $967 | 7-10 days | 7-10 days | |
| 100 μg | $1,390 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Biotinylated Human CD73, His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 2.5 ng/ml determined by ELISA. |
| Description | CD73 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is P21589-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P21589-1 |
| Construction | Trp27-Lys547 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73) |
| Research Background |
| Molecular Weight | 60.92 kDa (Predicted); 60-70 kDa (Due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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