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Synonyms: NET, Hek6, EPHT2, EPH receptor B1, ELK

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $108 | 7-10 days | 7-10 days | |
| 10 μg | $178 | 7-10 days | 7-10 days | |
| 20 μg | $296 | 7-10 days | 7-10 days | |
| 50 μg | $696 | 7-10 days | 7-10 days |
| Bioactivity | 1. Immobilized Human EphB1 His at 2 μg/mL (100 μL/well) can bind Human Ephrin-B1 His & hFc, the EC50 of Human Ephrin-B1 His & hFc is 35-200 ng/mL.
2. Immobilized Human EphB1 His at 2 μg/mL (100 μL/well) can bind Human Ephrin B2 His & hFc, the EC50 of Human Ephrin B2 His & hFc is 1.5-9.0 ng/mL. |
| Description | EphB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60 kDa and the accession number is P54762-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P54762-1 |
| Construction | A DNA sequence encoding the human EPHB1 (P54762-1) extracellular domain (Met 1-Pro 540) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Met 18 |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4, 5% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | NET, Hek6, EPHT2, EPH receptor B1, ELK |
| Research Background |
| Molecular Weight | 60 kDa (predicted); 60 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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