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Anti-GSN Antibody (4G383)

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Catalog No. TMAH-00518

Anti-GSN Antibody (4G383) is an antibody targeting GSN. Anti-GSN Antibody (4G383) can be used in ELISA, WB, IF.

Anti-GSN Antibody (4G383)

Anti-GSN Antibody (4G383)

😃Good
Catalog No. TMAH-00518
Anti-GSN Antibody (4G383) is an antibody targeting GSN. Anti-GSN Antibody (4G383) can be used in ELISA, WB, IF.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF

Reactivity: Human, Mouse
Ig Type
Rabbit IgG
Clone
4G383
Reactivity
Human, Mouse
Verified Activity
1. Western Blot
-Positive WB detected in: U251 whole cell lysate, MCF7 whole cell lysate
-All lanes: GSN antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 85 kDa
-Observed band size: 85 kDa
2. Immunofluorescence staining of hela cell with TMAH-00518 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Immunofluorescence staining of NIH/3T3 cell with TMAH-00518 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
ELISA, WB, IF
Recommended Dose
WB:1:1000-1:5000; IF:1:20-1:200.
Antibody Type
Monoclonal
Subcellular Localization[Isoform 2]: Cytoplasm, cytoskeleton.; [Isoform 1]: Secreted.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundCalcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human GSN Protein
Antigen Species
Human
Gene ID
2934
Uniprot ID
Biology Area
Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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