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Anti-GSN Antibody (4G383) is an antibody targeting GSN. Anti-GSN Antibody (4G383) can be used in ELISA, WB, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human, Mouse |
Ig Type | Rabbit IgG |
Clone | 4G383 |
Reactivity | Human, Mouse |
Verified Activity | 1. Western Blot -Positive WB detected in: U251 whole cell lysate, MCF7 whole cell lysate -All lanes: GSN antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 85 kDa -Observed band size: 85 kDa 2. Immunofluorescence staining of hela cell with TMAH-00518 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 3. Immunofluorescence staining of NIH/3T3 cell with TMAH-00518 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:1000-1:5000; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | [Isoform 2]: Cytoplasm, cytoskeleton.; [Isoform 1]: Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human GSN Protein |
Antigen Species | Human |
Gene ID | 2934 |
Uniprot ID | |
Biology Area | Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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