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Anti-acnB Antibody-HRP (6F295)
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Catalog No. TMAY-02484H
Alias yacJ, yacI
Anti-acnB Antibody-HRP (6F295) is a HRP-conjugated Mouse antibody targeting acnB. Anti-acnB Antibody-HRP (6F295) can be used in ELISA.
Anti-acnB Antibody-HRP (6F295)
Anti-acnB Antibody-HRP (6F295)
😃Good
Catalog No. TMAY-02484HAlias yacJ, yacI
Anti-acnB Antibody-HRP (6F295) is a HRP-conjugated Mouse antibody targeting acnB. Anti-acnB Antibody-HRP (6F295) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μg | $116 | 7-10 days | |
| 100 μg | $293 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA Reactivity: E. coli |
| Alias | yacJ, yacI |
| Ig Type | Mouse IgG2a |
| Clone | 6F295 |
| Reactivity | E. coli |
| Application | ELISA |
| Recommended Dose | ELISA: 0.1-1 μg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Escherichia coli acnB ( TMPY-02375; P36683; Met1-Val865). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases. |
| Conjucates | HRP |
| Others Formats | Unconjugated |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Escherichia coli acnB (TMPY-02375) |
| Antigen Species | E. coli |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. Keep away from direct sunlight. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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