Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, IF, FCM

Reactivity: Human

Rabbit IgG

4A515

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate(30µg), JK whole cell lysate(30µg), SY5Y whole cell lysate(30µg)
-All lanes: LDLR antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/20000 dilution
-Predicted band size: 96 kDa
-Observed band size: 100,140 kDa
-Exposure time: 60s
2. Immunofluorescence staining of Hela cell with TMAH-00688 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
4. Immunofluorescence staining of HepG2 cell with TMAH-00688 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
5. Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
6. Overlay Peak curve showing Hela cells stained with TMAH-00688 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, IHC, IF, FCM

IHC:1:50-1:200; IF:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human LDL Receptor

Human

3949

Cancer, Cardiovascular, Metabolism, Signal transduction