Anti-Caspase-3 Antibody (8K50) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (8K50) can be used in ELISA, WB, IHC, IF.

Rabbit IgG

8K50

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate
-All lanes: pro Caspase 3 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 32 kDa
-Observed band size: 32 kDa
2. IHC image of TMAH-00139 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of Hela Cells with TMAH-00139 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, WB, IHC, IF

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthesized peptide derived from human pro Caspase 3

Human

836

Cancer, Cell biology, Metabolism