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Anti-BCAP31 Antibody (3E493)
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Catalog No. TMAH-00105
Alias Protein CDM, p28, DXS1357E, BCR-associated protein 31, B-cell receptor-associated protein 31, BCAP 31, Bap31, 6C6-AG tumor-associated antigen
Anti-BCAP31 Antibody (3E493) is an antibody targeting BCAP31. Anti-BCAP31 Antibody (3E493) can be used in ELISA, WB, IHC, IF, FCM.
Anti-BCAP31 Antibody (3E493)
Anti-BCAP31 Antibody (3E493)
😃Good
Catalog No. TMAH-00105Alias Protein CDM, p28, DXS1357E, BCR-associated protein 31, B-cell receptor-associated protein 31, BCAP 31, Bap31, 6C6-AG tumor-associated antigen
Anti-BCAP31 Antibody (3E493) is an antibody targeting BCAP31. Anti-BCAP31 Antibody (3E493) can be used in ELISA, WB, IHC, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IF, FCM Reactivity: Human |
| Alias | Protein CDM, p28, DXS1357E, BCR-associated protein 31, B-cell receptor-associated protein 31, BCAP 31, Bap31, 6C6-AG tumor-associated antigen |
| Ig Type | Rabbit IgG |
| Clone | 3E493 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate -All lanes: BCAP31 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 28, 35 kDa -Observed band size: 28-36 kDa 2. IHC image of TMAH-00105 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of A549 cell with TMAH-00105 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 4. Overlay Peak curve showing PC3 cells stained with TMAH-00105 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, IHC, IF, FCM |
| Recommended Dose | WB:1:500-1:2000; IHC:1:50-1:200; IF:1:50-1:200; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40. In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2. May be involved in CASP8-mediated apoptosis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human BCAP31 |
| Antigen Species | Human |
| Gene ID | 10134 |
| Uniprot ID | |
| Biology Area | Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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