1.Loaded Biotinylated Recombinant Human VEGFR1/FLT1 Protein, His & Avi Tag (Cat#TMPY-06780) on SA Biosensor, can bind Recombinant VEGF165 Protein, Human, Cynomolgus with an affinity constant of 0.506 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
2.Immobilized Recombinant Human VEGFR1/FLT-1 Protein (His & Avi Tag), Biotinylated (Cat#TMPY-06780) at 2 μg/mL (100 μL/well) on Streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human/Cynomolgus VEGF/VEGFA/VEGF165 Protein, the EC50 is 58-174 ng/mL(Routinely tested).
3.Immobilized Recombinant Human/Cynomolgus VEGF/VEGFA/VEGF165 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human VEGFR1/FLT-1 Protein (His & Avi Tag), Biotinylated (Cat#TMPY-06780), the EC50 is 8-30 ng/mL (QC tested).

FLT1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 85.45 kDa and the accession number is P17948-1.

Human

HEK293 Cells

VEGFR-1,VEGFR1,fms-related tyrosine kinase 1,FLT-1,FLT

A DNA sequence encoding the Human FLT1 (P17948-1) (Met1-Asn756) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ser 27

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains sevenIg-like C2-type (immunoglobulin-like) domains and oneprotein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy