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SARS-CoV-2 Spike S1 Protein (HV69-70 deletion & N439K & D614G, His)

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Synonyms:

Catalog No. TMPY-06093 Copy Product Info
SARS-CoV-2 Spike S1 Protein (HV69-70 deletion & N439K & D614G, His) is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.2 kDa and the accession number is A0A6G7K2L4.

Product Introduction

Bioactivity
Bioactivity
Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1(HV69-70 deletion, N439K, D614G)-His Recombinant Protein, the EC50 of SARS-CoV-2 Spike S1(HV69-70 deletion, N439K, D614G)-His Recombinant Protein is 60-360 ng/mL.
Description
SARS-CoV-2 Spike S1 Protein (HV69-70 deletion & N439K & D614G, His) is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.2 kDa and the accession number is A0A6G7K2L4.
Species
SARS-CoV-2
Expression System
HEK293 Cells
TagC-His
Accession NumberA0A6G7K2L4
ConstructionA DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, N439K, D614G)-His Recombinant Protein (YP_009724390.1) (Met1-Arg685(HV69-70 deletion, N439K, D614G)) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Val 16
Protein Purity
> 90 % as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
Chemical Properties
Molecular Weight76.2 kDa (predicted); 105.8 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: SARS-CoV-2 Spike S1 Protein (HV69-70 deletion & N439K & D614G, His) chemical structure | SARS-CoV-2 Spike S1 Protein (HV69-70 deletion & N439K & D614G, His) molecular weight