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Anti-p21 Polyclonal Antibody
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Catalog No. TMAB-01312
Alias Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1
Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-p21 Polyclonal Antibody
Anti-p21 Polyclonal Antibody
😃Good
Catalog No. TMAB-01312Alias Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1
Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1 |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p21) Polyclonal Antibody, Unconjugated (TMAB-01312) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Huvec (Human) Cell Lysate at 30 μg Lane 4: U87MG (Human) Cell Lysate at 30 μg Lane 5: 293T (Human) Cell Lysate at 30 μg Lane 6: A673 (Human) Cell Lysate at 30 μg Primary: Anti-p21 (TMAB-01312) at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:20-100; IHC-Fr: 1:20-100; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic and Nuclear. |
| Tissue Specificity | Expressed in all adult tissues, with 5-fold lower levels observed in the brain. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. |
| Immunogen | Recombinant Protein: human p21 |
| Antigen Species | Human |
| Gene Name | CDKN1A |
| Gene ID | |
| Protein Name | Cyclin-dependent kinase inhibitor 1 |
| Uniprot ID | |
| Biology Area | Cip / Kip,Cip/Kip,DNA / RNA binding,Co-activators/co-repressors,Zinc Finger,Co-activators/co-repressors |
| Function | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. |
| Molecular Weight | Theoretical: 18 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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