Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-p21 Polyclonal Antibody

😃Good
Catalog No. TMAB-01312
Alias Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1

Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-p21 Polyclonal Antibody

Anti-p21 Polyclonal Antibody

😃Good
Catalog No. TMAB-01312Alias Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1
Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasWildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p21) Polyclonal Antibody, Unconjugated (TMAB-01312) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Lane 3: Huvec (Human) Cell Lysate at 30 μg
Lane 4: U87MG (Human) Cell Lysate at 30 μg
Lane 5: 293T (Human) Cell Lysate at 30 μg
Lane 6: A673 (Human) Cell Lysate at 30 μg
Primary:
Anti-p21 (TMAB-01312) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:20-100; IHC-Fr: 1:20-100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic and Nuclear.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.
Antigen Details
Immunogen
Recombinant Protein: human p21
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cip / Kip,Cip/Kip,DNA / RNA binding,Co-activators/co-repressors,Zinc Finger,Co-activators/co-repressors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-p21 Polyclonal Antibody | purchase Anti-p21 Polyclonal Antibody | Anti-p21 Polyclonal Antibody cost | order Anti-p21 Polyclonal Antibody