Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-PIK3CA Antibody (9D297)
😃Good
Catalog No. TMAH-00978
Anti-PIK3CA Antibody (9D297) is an antibody targeting PIK3CA. Anti-PIK3CA Antibody (9D297) can be used in ELISA, WB, IF.
Anti-PIK3CA Antibody (9D297)
Anti-PIK3CA Antibody (9D297)
😃Good
Catalog No. TMAH-00978
Anti-PIK3CA Antibody (9D297) is an antibody targeting PIK3CA. Anti-PIK3CA Antibody (9D297) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | 196 € | 7-10 days | |
| 100 μL | 329 € | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PIK3CA Antibody (9D297) is an antibody targeting PIK3CA. Anti-PIK3CA Antibody (9D297) can be used in ELISA, WB, IF. |
| Ig Type | Rabbit IgG |
| Clone | 9D297 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate -All lanes: PIK3CA antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 125 kDa -Observed band size: 110 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-00978 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others. Plays a role in the positive regulation of phagocytosis and pinocytosis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human PI 3 Kinase catalytic subunit alpha |
| Antigen Species | Human |
| Gene ID | 5290 |
| Uniprot ID | |
| Biology Area | Cancer, Immunology, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Serine/threonine protein kinase PIK3CAPtdIns-3-kinase subunit αPtdIns-3-kinase subunit alphaPtdIns-3-kinase subunit aPIK3CAPI3K-αPI3KαPI3-kinase subunit αPI3-kinase subunit alphaPI3-kinase subunit aPI3K-alphaPI3KalphaPI3K-aPI3KaPhosphoinositide-3-kinase catalytic α polypeptidePhosphoinositide-3-kinase catalytic alpha polypeptidePhosphoinositide-3-kinase catalytic a polypeptidePhosphoinositide 3-kinase αPhosphoinositide 3-kinase alphaPhosphoinositide 3-kinase aPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit α isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit a isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit α (PtdIns-3-kinase subunit p110-α;p110α)Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha;p110alpha)Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit a (PtdIns-3-kinase subunit p110-a;p110a)p110αp110alphap110ap110 αp110 alphap110 a5-bisphosphate 3-kinase catalytic subunit α isoform5-bisphosphate 3-kinase catalytic subunit alpha isoform5-bisphosphate 3-kinase catalytic subunit a isoform
Preview
Related Tags: buy Anti-PIK3CA Antibody (9D297) | purchase Anti-PIK3CA Antibody (9D297) | Anti-PIK3CA Antibody (9D297) cost | order Anti-PIK3CA Antibody (9D297)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.