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Anti-Firefly Luciferase Antibody (8U71) is a Mouse antibody targeting Firefly Luciferase. Anti-Firefly Luciferase Antibody (8U71) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $182 | 7-10 days | 7-10 days | |
| 500 μL | $756 | 7-10 days | 7-10 days |
| Description | Anti-Firefly Luciferase Antibody (8U71) is a Mouse antibody targeting Firefly Luciferase. Anti-Firefly Luciferase Antibody (8U71) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Synonyms | Luciferin 4 monooxygenase, Firefly Luciferase Luciferase, Firefly, EC 1.13.12.7 |
| Ig Type | IgG1 |
| Clone | 8U71 |
| Reactivity | (predicted:Firefly) |
| Application | |
| Recommended Dose | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:500-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Peroxisome. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
| Immunogen | Luciferase comes from the North American firefly (Firefly) |
| Gene Name | Luciferase |
| Protein Name | Luciferin 4-monooxygenase |
| Biology Area | Luciferase |
| Function | Produces green light with a wavelength of 562 nm. |
| Molecular Weight | Theoretical: 61 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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