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HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 μg | $55 | 7-10 days | 7-10 days | |
| 10 μg | $88 | 7-10 days | 7-10 days | |
| 25 μg | $182 | - | In Stock | |
| 50 μg | $285 | 7-10 days | 7-10 days | |
| 100 μg | $480 | 7-10 days | 7-10 days | |
| 500 μg | $2,050 | 7-10 days | 7-10 days | |
| 1 mg | $2,730 | 7-10 days | 7-10 days |
| Biological Activity | Immobilized Human HGF R, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.1 ng/ml determined by ELISA. (QC Test) Immobilized Human HGF R, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA. |
| Description | HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P08581-1 |
| Synonyms | Tyrosine-protein kinase Met,SF receptor,Proto-oncogene c-Met,MET,HGF/SF receptor,HGF receptor,Hepatocyte growth factor receptor |
| Construction | Glu25-Thr932 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC |
| Molecular Weight | 32.5 kDa (α chain) and 72.1 kDa (β chain) (Predicted); 45-60 kDa (α subunit) and 80-100 kDa (β subunit) (Reducing conditions due to glycosylation) |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. |
| Research Background | c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. |
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