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HGFR/c-Met Protein, Human, Recombinant (His) V2

TargetMol | SPR
Catalog No. TMPY-01296U

HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1.

HGFR/c-Met Protein, Human, Recombinant (His) V2

HGFR/c-Met Protein, Human, Recombinant (His) V2

TargetMol | SPR
Catalog No. TMPY-01296U
HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
5 μg$557-10 days7-10 days
10 μg$887-10 days7-10 days
25 μg$182-In Stock
50 μg$2857-10 days7-10 days
100 μg$4807-10 days7-10 days
500 μg$2,0507-10 days7-10 days
1 mg$2,7307-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]
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Product Information

Biological Activity
Immobilized Human HGF R, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.1 ng/ml determined by ELISA. (QC Test)
Immobilized Human HGF R, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA.
Description
HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP08581-1
Synonyms
Tyrosine-protein kinase Met,SF receptor,Proto-oncogene c-Met,MET,HGF/SF receptor,HGF receptor,Hepatocyte growth factor receptor
Construction
Glu25-Thr932
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Molecular Weight32.5 kDa (α chain) and 72.1 kDa (β chain) (Predicted); 45-60 kDa (α subunit) and 80-100 kDa (β subunit) (Reducing conditions due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

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