Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-RhoA Antibody (1W191)
😃Good
Catalog No. TMAY-02348
Alias RHOH12, RHO12, ras homolog family member A, ARHA, ARH12
Anti-RhoA Antibody (1W191) is a Mouse antibody targeting RhoA. Anti-RhoA Antibody (1W191) can be used in ELISA,FCM,ICC/IF.
Anti-RhoA Antibody (1W191)
Anti-RhoA Antibody (1W191)
😃Good
Catalog No. TMAY-02348Alias RHOH12, RHO12, ras homolog family member A, ARHA, ARH12
Anti-RhoA Antibody (1W191) is a Mouse antibody targeting RhoA. Anti-RhoA Antibody (1W191) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, FCM, ICC/IF Reactivity: Human |
| Alias | RHOH12, RHO12, ras homolog family member A, ARHA, ARH12 |
| Ig Type | Mouse IgG2a |
| Clone | 1W191 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human RHOA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human RHOA monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-RHOA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; TMPY-01958; P61586-1; Met 1-Leu 193). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. |
| Conjucates | Unconjugated |
| Others Formats | PE |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Human RhoA protein (TMPY-01958) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-RhoA Antibody (1W191) | purchase Anti-RhoA Antibody (1W191) | Anti-RhoA Antibody (1W191) cost | order Anti-RhoA Antibody (1W191)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.